| Erfa Biotech |
| Mouse monoclonal antibody to cardiac myosin light chain 1 (8-3 F6) |
| Mouse monoclonal antibody to cardiac myosin light chain 1 (F5) |
| Mouse monoclonal antibody to cardiac myosin heavy chain (3-48) |
| Sheep
polyclonal antiserum to human ventricular myosin light chain 1 |
Mouse monoclonal antibody
to cardiac myosin heavy chain
(FOR
RESEARCH)
Hybridoma designation: 3-48
The antibody was obtained from polyethylene glycol fusion of non-secreting mouse
myeloma(P3X63.Ag8.653) cells with spleen cells from Balb/c mouse immunized with
human ventricular myosin.
ANTIBODY CHARACTERISTICS
| Immunoglobulin class : | IgG1,
k |
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| Isoelectric point : | pH
7.35-6.55 |
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| Molecular weight of IgG : |
according
to SDS-PAGE under mercaptoethanol (reduced
conditions) 153.8
kd
|
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| Molecular weight of F(ab')2 : | according
to SDS-PAGE under mercaptoethanol (reduced
conditions) 116
kd heavy chain: 30.8 kd light chain: 27.2 kd |
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| Antibody production : | Clinical
grade tissue culture supernatant was concentrated and purified to
homogeneity by a series of chromatographic steps. Immunoglobulin was then
fragmented to F(ab')2 by proteolytic degradation and reduced to Fab' by
cysteine. |
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| Kit preparation : | Pure
3-48 Fab' fragments were "pre-tinned", excess tin removed and
antibody fragments fromulated in the presence of technetium reducing agent
into sterile hypovials, and lyophilized. For radiolabelling, vial content is reconstituted with 99mTc freshly eluted from the generator and after 30 minute incubation at room temperature is ready. |
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| Affinity constants for : | Antigen | IgG | Fab' | ||
| Human ventricular myosin heavy chains | 3.33 x 108M-1 | ||||
| Human atrial myosin heavy chains | 1.48 x 108M-1 | ||||
| Human skeletal muscle myosin | 1.06 x 108M-1 | ||||
| Canine
cardiac myosin heavy chains |
2.06 x 108M-1 | 1.2 x 107M-1 | |||
| Immunologic specificity : | The
monoclonal antibody is myosin alpha and beta heavy chain specific. On Western
blots and in ELISA it reacts with purified heavy chains of atrial and ventricular
myosin isolated from human, canine, bovine, porcine, murine, rabbit, and
rat cardiac tissues. It binds to A-band of thick filament of cardiac and
skeletal muscle myosin as determined by indirect immunofluorescence on cryocuts,
and indirect immunoperoxidase staining of Carnoy fixed tissues. It stains
human and canine myocytes in culture as determined by indirect immunofluorescence. |
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| Cross-reactivity : | 3-48
antibody cross-reacts with heavy chains of human myosin isolated from:
slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal
M5 peptide. It does not react with human platelet, smooth muscle myosin
heavy chains, myosin light chains, myosin fragments circulating in blood
of patients with myocardial infarction, nor does it react with any other
human tissue antigens or blood cells. |
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| Storage : | Store
at 4oC. |
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| Stability : | IgG | When reconstituted in aqueous solution IgG remains stable for six months if stored at 4oC. No considerable loss of activity was detected when stored at -70oC over a period of two years. | |||
| F(ab')2 | When
reconstituted in aqueous solution F(ab')2 remains stable for
six months if stored at 4oC. No considerable loss of activity was detected when stored at -20oC over a period of one year. |
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| CVTM | Stable
for one year when stored lyophilized at 4oC. |
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| Delivery form : | Lyophilized |
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| Delivery quantities : | -
1 mg/vial SKU #: 76250 - 5 mg/vial SKU #: 76255 |
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