| Erfa Biotech |
| Carcinoembryonic antigen |
| Monoclonal antibody to carcinoembryonic antigen |
| Monoclonal syngeneic anti-idiotypic antibody to anti-carcinoembryonic antigen monoclonal antibody |
Mouse monoclonal syngeneic
anti-idiotypic antibody to anti-carcinoembryonic antigen monoclonal antibody
(FOR
RESEARCH)
TRADE NAME: L12E2
Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly
typical, high-molecular weight, cell surface glycoprotein over-expressed in
adenocarcinomas of entero-dermally-derived organs of the digestive system and
in fetal colon. Highly specific anti-CEA mouse monoclonal antibody was produced
as a result of a unique immunization protocol.
ANTIBODY CHARACTERISTICS
| Production : | The
antibody was obtained from the polyethylene glycol fusion of non-secreting
mouse myeloma (P3X63.Ag8.653) cells with popliteal lymph node lymphocytes
from BALB/c mice immunized with the anti-CEA monoclonal antibody C234
mAb. |
| Purification : | Tissue
culture derived antibody was precipitated with ammonium sulfate and
affinity chromatographed on CNBr-activated Separose 4B-C234
mAb. |
| Immunologic class : | IgG1
k |
| Immunologic specificity : | L12E2
mAb is specific fot the immunizing idiotope of C234 mAb. It
binds C234 mAb at the CEA binding site since : (a) it inhibits the binding of CEA to C234 mAb as well as, (b) C234 binding to CEA in ELISA. Binding of L12E2 to its corresponding C234 could be inhibited by CEA. |
| Potential use : | L12E2
anti-idiotypic antibody could be used for: 1. Affinity purification of anti-CEA antibodies recognizing the same C234 epitope on CEA 2. Active immunization as a surrogate of CEA 3. Idiotype identification |
| Storage : | Store
at 4oC. |
| Stability : | When
reconstituted in aqueous solution IgG remains stable for six months if
stored at 4oC. No considerable loss of activity was detected
when stored at -20oC over a period of six months. |
| Delivery form : | Lyophilized
pure IgG |
| Delivery quantities : |
1 mg/vial SKU #:76150 |